11-13 December 2019
ILL4
Europe/Paris timezone

A multi-technique approach for the characterization of the self-assembling of cyclic peptides into nanotubes at biological model membranes

12 Dec 2019, 10:20
20m
Oral presentation Session C

Speaker

Barbara Claro (CIQ-UP Dep. Chemistry & Biochemistry, FCUP, Porto, Portugal)

Description

Bacterial resistance is presently a major public health concern, due to excessive and misuse of antibiotics. This has stressed the research on new antibiotics with new mechanisms of action [1]. Antimicrobial peptides are part of our innate immune system and represent a new antibiotic paradigm, as they aim the bacterial membrane, have been studied in the past decade [2]. Within this research effort, a new class of potential antimicrobial peptides has emerged [3] - Cyclic Peptides (CP) with an even number of alternating D and L-α-amino acids that assume a planar conformation and form the active species, Self-assembling Cyclic Peptide Nanotubes (SCPNs), when in contact with bacterial membranes. We used different biophysical experimental techniques (DSC, Fluorescence, solid state NMR, ATR-FTIR) together with an in-silico approach to characterize the interaction of these antimicrobial SCPNs with different model membranes, aiming ultimately at unveiling their possible mechanism of action.
Results from these techniques will be shown and compared, allowing us to characterize the formation and orientation of SCPNs at different membranes, and to discriminate the most important factors ruling these peptides/membrane interactions.

Acknowledgement: FCT, Portugal UID/QUI/0081/2013, IF/01133/2015, POCI-01-0145-FEDER-030579, PD/BD/135095/2017, and FEDER (COMPETE 2020) for Norte-01-0145-FEDER-000028
References
1 L. Fernández, R. E. W. Hancock, Clin. Microbiol. Rev. 25 (2012) 661-681
2. T. Silva, B. Claro et al Langmuir 34 (2018) 2158-2170
3 S. Fernandez-Lopez et al Nature 412 (2001) 452–455

Primary author

Barbara Claro (CIQ-UP Dep. Chemistry & Biochemistry, FCUP, Porto, Portugal)

Co-authors

Eva Freire (CIQUS Singular Research Centre in Chemical Biology and Molecular Materials, USC, Santiago de Compostela, Spain) Juan Granja (CIQUS Singular Research Centre in Chemical Biology and Molecular Materials, USC, Santiago de Compostela, Spain) Erik Goormaghtigh (3 Université Libre de Bruxelles, Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, Bruxelles, Belgium) Tiago Ferreira (NMR – Institute for Physics, Martin-Luther University Halle-Wittenberg, Germany) Rebeca Garcia-Fandiño (CIQUS Singular Research Centre in Chemical Biology and Molecular Materials, USC, Santiago de Compostela, Spain) Margarida Bastos (CIQ-UP Dep. Chemistry & Biochemistry, FCUP, Porto, Portugal)

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