11-13 December 2019
ILL4
Europe/Paris timezone

DNA-tagged lipid bilayers: novel nano-scaled membrane-mimetic systems

12 Dec 2019, 10:00
20m
Oral presentation Session C

Speaker

Karim Fahmy (Helmholtz-Zentrum Dresden-Rossendorf)

Description

Lipid bilayers and lipid-associated proteins play a crucial role in biology. Since studies and manipulation in vivo are inherently challenging, several in vitro membrane-mimetic systems have been developed to enable the study of lipidic phases, lipid-protein interactions and membrane protein function. Controlling the size and shape or introducing functional elements in a programmable way is, however, difficult to achieve with common systems based on polymers, peptides or membrane scaffolding proteins. We have combined DNA-nanotechnology with lipid bilayer self-assembly to create DNA-encircled bilayers (DEBs) as a novel nano-scaled membrane-mimetic. For this, alkylated oligonucleotides were hybridized to a single-stranded minicircle (ssMC) to provide an inner hydrophic surface for lipid attachment. DEBs open new routes to membrane biophysical studies, enabling improved size control, stability and programmability[1]. Here, we present further developments of DNA-associated lipid bilayers for the reconstitution of membrane proteins and for the generation of self-assembled higher order structures. The latter may be ultimately used for the structural biology of membrane proteins in Cryo-EM or in diffraction experiments using advanced X-ray sources, such as synchrotrons or XFELs.

  1. K. Iric, M. Subramanian, et al. Nanoscale 10 (2018) 18463-18467

Primary authors

Madhumalar Subramanian (Helmholtz-Zentrum Dresden Rossendorf) Jana Oertel (Helmholtz-Zentrum Dresden-Rossendorf) Karim Fahmy (Helmholtz-Zentrum Dresden-Rossendorf)

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