16-17 March 2020
ILL4
Europe/Paris timezone

Assembly of membrane protein complexes with encapsulated lipids

Not scheduled
15m
ILL4-rdc-1 - Amphi Chadwick (ILL4)

ILL4-rdc-1 - Amphi Chadwick

ILL4

110
Oral Session 4

Speaker

Ivan Gushchin (Moscow Institute of Physics and Technology)

Description

Recent breakthroughs in X-ray crystallography, Cryo-EM and complementary approaches resulted in elucidation of many new structures of membrane proteins (MPs) and their complexes. Several classes of MPs, such as microbial rhodopsins, rotary ATPase subunits c, or light harvesting complexes 2, form ring-like assemblies with several lipid molecules trapped inside. Whereas the proteins are usually well resolved in experimental structures, the lipids are more disordered and cannot be readily modeled; the number and nature of the trapped molecules is also often not clear. This is problematic, because the trapped lipids cannot exchange with the pool of the surrounding membrane lipids in simulations, and the errors in the starting models can not correct themselves, as often happens in normal molecular dynamics simulations of soluble and membrane proteins. To address this problem, we simulated the process of the assembly of the MP-lipid complex explicitly, and then removed the excess lipids to achieve a very good fit to the noisy experimental densities that are available [1]. The presented approach is expected to be generalizable to all MP complexes with encapsulated lipid patches. Besides producing molecular models of MP-lipid complexes, the approach also highlights intriguing adaptations of ring-forming MPs that promote efficient assembly.

  1. Novitskaia, O.; Buslaev, P.; Gushchin, I. Assembly of Spinach Chloroplast ATP Synthase Rotor Ring Protein-Lipid Complex. Front. Mol. Biosci. 2019, 6.

Primary author

Ivan Gushchin (Moscow Institute of Physics and Technology)

Co-authors

Dr. Pavel Buslaev (Nanoscience Center, Department of Chemistry, University of Jyväskylä, Jyväskylä, Finland) Mr. Alexander Kuzmin (Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia) Mr. Ilya Sergeev (Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia)

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