Mar 16 – 17, 2020
Europe/Paris timezone

Cryo-Electron Tomography for in situ Structural Biology

Not scheduled
ILL4/rdc-1 - Amphi Chadwick (ILL4)

ILL4/rdc-1 - Amphi Chadwick


Oral Session 9


Mr Alister Burt (Institut de Biologie Structurale)


Short Abstract

Cryo-electron tomography (cryo-ET) is a technique which allows direct visualisation of vitrified biological samples in 3D. In a framework conceptually similar to single particle analysis in cryo-EM, it is possible to achieve high-resolution (subnanometer and beyond!) insights into macromolecules directly within their cellular context by alignment and averaging of multiple copies of the same macromolecule. While possible, obtaining such results requires specialist knowledge of a complex workflow and as such is not routinely achieved in many labs worldwide.

Talk Summary

  • Overview of cryo-electron tomography
  • Recent results
  • Challenges in data processing
  • Recent algorithmic developments
  • New possibilities?

Primary authors

Mr Alister Burt (Institut de Biologie Structurale) Dr Irina Gutsche (Institut de Biologie Structurale)

Presentation materials

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