CANCELLED : Algorithms for integrative structural biology

Session

Session 9

17 Mar 2020, 13:30

ILL4/rdc-1 - Amphi Chadwick (ILL4)

12. Cryo-Electron Tomography for in situ Structural Biology

Mr Alister Burt (Institut de Biologie Structurale)

Oral

Short Abstract

Cryo-electron tomography (cryo-ET) is a technique which allows direct visualisation of vitrified biological samples in 3D. In a framework conceptually similar to single particle analysis in cryo-EM, it is possible to achieve high-resolution (subnanometer and beyond!) insights into macromolecules directly within their cellular context by alignment and averaging of multiple...

24. General thoughts and Examples for EM fitting: an historical perspective.

Dr Pablo Chacon

Oral

In last the decades, significant progress was made by combining cryo-EM data with high-resolution structures. We review our adventure developing computational fitting techniques to interpret EM data in terms of available atomic structures. We will try to illustrate the power and limitations of our fitting tools with key examples.

8. Simultaneous determination of protein structure and dynamics using cryo-electron microscopy

Dr Massimiliano Bonomi (Institut Pasteur - CNRS)

Oral

Cryo-electron microscopy is rapidly emerging as a powerful technique to determine the structures of complex macromolecular systems elusive to other techniques. Since many of these systems are highly dynamical, characterising also their movements is a crucial step to unravel their biological functions. In this talk, I will present an integrative modelling approach to simultaneously determine...