Feb 12 – 16, 2024
EPN Campus
Europe/Paris timezone

Detailed analysis of a thermostable protein-DNA complex: the case of Sac7d as a prototype for protein-DNA interaction

Not scheduled
30m
IBS seminar room (EPN Campus)

IBS seminar room

EPN Campus

71 avenue des Martyrs 38000 Grenoble
Poster

Speaker

Elena Alvarez Sanchez (Nantes Université, US2B, CNRS, UMR6286 & Affilogic, Nantes, France)

Description

Sac7d is a 7kDa protein belonging to the class of the small chromosomal proteins
from archeon Sulfolobus acidocaldarius. Sac7d was discovered in 1974 in Yellowtone
National Parks geysers, and studied extensively since then for its remarkable
stability at large pH and temperature ranges. Sac7d binds to DNA minor groove to
protect DNA from these extreme conditions by increasing its melting temperature.
In this study, we analyzed the Sac7d-DNA complex using 1 µs molecular dynamics
simulations. The interaction energy ofthe interface was decomposed using Molecular Mechanics Generalized Born Surface Area (MM/GBSA) to determine at the amino acids level which residue contributed most to DNA binding. Twelve amino acids are essential for DNA binding, out of which three were not identified previously by former studies.
One of these new aminoacids, R63, may be involved in a dynamic protein-DNA interaction. Our analysis provides novel insights into how the Sac7d chaperones allows to protect DNA from degradation in extreme conditions.

Submitting to: 8th CAPRI assessment meeting

Primary author

Elena Alvarez Sanchez (Nantes Université, US2B, CNRS, UMR6286 & Affilogic, Nantes, France)

Presentation materials

There are no materials yet.