Speaker
Description
Membrane proteins are responsible of all the communication between living cells and their environment. Despite their basic and therapeutic interest, their are under-represented in our structural biology knowledge due to the intrisic difficulties presented by their production, purification and handling. Nanodiscs are a great tools to study them in their native lipidic environment, and to study the effect of the variation of this environment on their structure. In particular, BioSANS can make use of contrast variation in this aim. Here, as an example, we will present how the contrast variation can be used to study the nanodiscs formation process.Building on the pioneering work of Maric et al. our goal is to provide our users with adequat nanodiscs constituants, at required deuteration levels, to enable the SANS study of membrane protein conformation and the interplay with their surrounding lipidic environment.
