GEM2023

Session

Structural biology

16 Mar 2023, 08:30

93. Structural biology in the outer membrane of living bacteria

Prof. Bart Hoogenboom (University College London)

16/03/2023, 08:30

Invited

The outer membrane is a crucial barrier that protects Gram-negative bacteria against harsh environments and restricts cellular entry for antibiotics. It consists of lipopolysaccharides (LPS) and phospholipids and is densely packed with outer membrane proteins. Yet it remains largely unknown how these are organised in the outer membrane, with various indications of non-homogeneous distributions...

68. Investigation of the Histidine Residues of the Growth Hormone Secretagogue Receptor by Solid-State NMR

Ms Emelyne Pacull (University of Leipzig)

16/03/2023, 09:10

Oral

The Growth Hormone Secretagogue Receptor (GHSR), a 366 amino acids Rhodopsin-like G proteins-coupled receptor (GPCRs), is involved in signal transduction into the cells and plays an important role in food intake, glucose metabolism and immune response. Its high constitutive activity of 50%, seldom among GPCRs, makes this receptor a particularly attractive pharmacological target. In the last...

48. Solid-state NMR investigation of the synergistic action of magainin antimicrobial peptides

Dr Ahmad Saad (University of Strasbourg)

16/03/2023, 09:30

Oral

Solid-state NMR investigation of the synergistic action of magainin antimicrobial peptides
Ahmad Saad, Jesus Raya, Elise Glattard, Burkhard Bechinger
Membrane Biophysics and NMR, Institut of Chemistry (UMR-7177), 1, rue Blaise Pascal Universite de Strasbourg/CNRS, 67000 Strasbourg, FRANCE

Abstract:
Magainin and PGLa are cationic, amphipathic antimicrobial peptides isolated from the skin of...

58. Model, structure and function of a bacterial virulence factor

Charline Mary (CNRS)

16/03/2023, 09:50

Oral

Bacterial virulence factors are essential for pathogens to disrupt host defense mechanisms and survive in the host. Our study focuses on a specific virulence factor, a membrane protein of unknown structure and function, common to several major bacterial pathogens.
First, combining structure prediction using Alphafold and evolutionary trace using Consurf has not only revealed an original fold...

41. Molecular-Level Architecture of a Microalgal Cell Wall

Dror WARSCHAWSKI (CNRS)

16/03/2023, 10:10

Oral

A. Poulhazan 1, A. A. Arnold 1, F. Mentink-Vigier 2, T. Wang 3, I. Marcotte 1, D. E. Warschawski 4

1 Department of Chemistry, University of Quebec at Montreal, Montreal, QC, Canada
2 National High Magnetic Field Laboratory, Tallahassee, FL, USA
3 Department of Chemistry, Michigan State University, East Lansing, MI, USA
4 Laboratoire des Biomolécules, CNRS UMR 7203,...

102. The puzzling activity of the antidepressant Vortioxetine at 5-HT3 receptors

Hugues Nury

16/03/2023, 14:00

Invited

Uriel López-Sánchez, Lachlan Munro, Anders S. Kristensen, Jacques Neyton, Guy Schoehn, Hugues Nury.

The serotonin 5-HT3 receptors are pentameric ligand-gated ion channels that play important roles in fast neurotransmission within the central and peripheral nervous systems. They are the targets of therapeutic compounds to treat chemotherapy-induced nausea, and also the targets of the...

83. Probing the coupled dynamics between lipids and membrane proteins in nanodiscs by high-pressure NMR spectroscopy

Ewen Lescop (CNRS)

16/03/2023, 14:40

Oral

Cell membranes represent a complex and variable environment in time and space of lipids and proteins. Their physico-chemical and functional properties are determined by lipid and protein components and their respective interactions. Here, we used NMR spectroscopy under hydrostatic pressure to study the close dynamic relationships between lipids and membrane proteins in ~10 nm nanodiscs. We...

85. DNA Origami-Based Protein Manipulation Systems : From structural biology to mechanical regulation

Dr Nesrine Aissaoui (Laboratoire CiTCoM, Faculté de Santé, Université Paris Cité, CNRS, Paris, 75006 France)

16/03/2023, 15:00

Oral

The introduction of DNA origami, which uses many staple strands to fold one long scaffold strand into a rationally designed nanostructure has dramatically improved the complexity and scalability of DNA nanostructures with unprecedented capabilities. Our goal is to explore the bottom-up structural DNA origami nanotechnology to build artificial molecular systems and machines sufficiently...

35. Cryo-em single particle density maps disclose protein-lipid association

Valerie Biou (laboratoire de biologie physico-chimique des protéines membranaires)

Oral

Membrane proteins reside at interfaces between aqueous and lipid media and solving their molecular structure relies most of the time on removing them from the membrane using detergent. Luckily, this solubilization process does not strip them from all the associated lipids and single-particle cryo-transmission electron microscopy (SP-TEM) has proved a very good tool to visualize both protein...

4. Structural biology

Prof. Bart Hoogenboom (University College London, London, UK), Prof. Hughes Nury (Institute of Structurale Biology, Grenoble, FR)