GEM2023

Session

Interaction lipids/polymers/membrane proteins

15 Mar 2023, 08:30

37. Unraveling the working mechanism of a tumor suppressor lipid

Prof. Joost Holthuis (Center for Cellular Nanoanalytics Osnabrueck (CellNanOS), University of Osnabrueck | )

15/03/2023, 08:30

Invited

Ceramides attract wide attention as tumor suppressor lipids that can act directly on mitochondria to trigger Bax-mediated cell death. While ceramide engagement in mitochondrial apoptosis is clinically relevant, molecular details of the underlying mechanism are largely unknown. A chemical screen for ceramide binding proteins combined with molecular dynamics simulations and functional studies in...

49. SANS for membrane proteins

Anne Martel (ILL)

15/03/2023, 09:10

Oral

Small angle neutron scattering enables to study the structure of macromolecular complexes in solution, at low resolution. Within last few years new tools dedicated to the study of membrane proteins were developed. An integrated Size exclusion chromatography (SEC, 1) system enables to exchange both the membrane protein detergent for its D2O-invisible counterpart (2, and the buffer for D2O-based...

32. Membrane permeabilization can be crucially biased by a fusogenic lipid composition: antimicrobial peptides and model membranes

Dr Maria Hoernke (Albert-Ludwigs-Universität Freiburg)

15/03/2023, 09:30

Oral

Induced membrane permeabilization or leakage is often taken as an indication for activity of a membrane-active effector molecule, such as antimicrobial peptides (AMPs) or their synthetic mimics. Nevertheless, it is poorly understood, what effect (or combination of effects) is actually required for antimicrobial activity.
What is often missing is the knowledge of the exact leakage mechanism as...

81. Spectrin βII suppresses endosomal vesicle trafficking by regulating membrane dynamics

Mr Daichi Kitagawa (Toyama university, Japan)

15/03/2023, 09:50

Oral

Spectrin is a cytoskeleton protein present in eukaryotic cells, contributing to membrane stability cooperating with actin and ankyrin. Our proteome analysis identified that Spectrin highly associates with Phosphatidylserine (PS) containing vesicles. However, the importance of the interaction between PS and Spectrin remains poorly understand. Therefore, we examined distribution of Spectrin βII,...

5. Interaction lipids/polymers/membrane proteins

Joost Holthius